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Includes:
- Lifetime access
- 5 hours of recorded videos.
- 9 Lectures
- No Certificate

To understand Biotechnology you must know basic concepts like techniques involved, troubleshooting and applications. A sound base of the theory involved in practical experimentation will help one excel in practical courses and develop interest in a research and teaching career if one wishes to.
This is an initial introductory online course, that will help you understand the basic techniques involved in cloning. It will familiarise a Class 10 or 12th Biology student or a B.Sc Biotechnology /Microbiology student with the principles of cloning. The focus will be on Plasmid isolation, Restriction digestion and Polymerase Chain Reaction (PCR).
To understand Biotechnology you must know basic concepts like techniques involved, troubleshooting and applications. A sound base of the theory involved in practical experimentation will help one excel in practical courses and develop interest in a research and teaching career if one wishes to.
This is an initial introductory online course, that will help you understand the basic techniques involved in cloning. It will familiarise a Class 10 or 12th Biology student or a B.Sc Biotechnology /Microbiology student with the principles of cloning. The focus will be on Plasmid isolation, Restriction digestion and Polymerase Chain Reaction (PCR).
Dr. Gautam Krishnan
Established Scientist & Professor of Biotechnology
I did my Phd from BITS PILANI (Topic-“ Functional Characterization of Recombinant α-crystallin of Mycobacterium tuberculosis H37Rv” ) after masters in Biological Sciences along with B Pharm.
From my school days I was interested in research and a Nature paper I studied in my 3rd year inspired me to take me a career of research especially directed towards health care
Molecular Biology: I am interested in studying about key proteins involved in disease especially antigenic proteins with the aim of creating new vaccines or diagnostics.
I am also interested in studying about the impact of immune status on response to bacterial pathogens especially secondary infections like in case of mycobacteria
Amongst these I would like to pursue research on gut mucosa and in the long term help develop theoretical models which could help diagnose key parameters that could help in global health care
My past research experience has been on malarial proteins involved in hemoglobin degradation with the aim of making enzymes as drug targets especially the proteases
I have also worked on anti bacterial broad spectrum antibiotics projects specifically on helicase E.coli Dna B which can be developed into a new drug target
I have also worked on a research project involved in scale up of process development for a diabetes product Insulin scaling up a recombinant process from gram scale to Kg scale.
Later on I worked on contract research processes for IgM and IgG purification for radiolabelled antibodies while setting up a new lab in Goa an wholly owned subsidiary of a US based CRO .
Sample of Projects Supervised:
(September 2006 – May 2009) Scientist I
(July 2001 – July 2006) Research Scientist
Administered the research & development team to develop new biological products of therapeutic value for the market with a team of 7 personnel
1) Title: To develop an industrial scale processes for making therapeutic proteins
Team Size: 7
Scope: The project was undertaken to develop lab scale process that could be scaled up to kg scale for production
Role: Semi preparative scale up (10gm scale) protein purification experiments
Validated batches of downstream processes to establish proof of concept & for toxicity testing.
Participated in campaign to make clinical grade material for testing
Process development & optimized key ion exchange & reverse phase HPLC Steps Impurity purification for submission to Quality Dept.
2) Title: To work on Contract Projects for customers working in the Drug Discovery Industry (Jul’01 –Apr’02)
Team Size: 3
Scope: The project was undertaken to study immunogenic proteins associated with cancer for expressing the protein in a soluble form
(January 1995 – July 2001) Research Assistant/ Research Associate
Worked on Drug Discovery programs for malaria & antibiotics with a team of 5 personnel Possess knowledge of Cloning, Expression & Enzymatic Assays of Bacterial Proteins.
Facilitated screening 20000 compounds for an Anti-Malarial Drug Discovery program and in developing Enzymatic Assays for Anti Malarial Drug Discovery program and Anti Bacterial program.
Project Handled:
1) Title: Anti-Malarial Project for development of Novel Anti-Malarial Drugs (Jan’95-Dec’99)
Team Size: 5
Scope: The project was undertaken to specify enzymes of malarial parasite, P. Falciparum with the idea of making rationally designed drugs. It was done by targeting development of an inhibitor against 3 protease – aspartic (Plasmepsin I, II) & cysteine (Plasmepsin II) of P. Falciparum
Role: Managed Cloning, Hyper expression of Proteases
Refolded E. Coli inclusion body pellets to send to Queensland
Pharmacological Research Institute, Australia to screen natural extracts anti malarial. Set up assays which can be adapted to medium and high throughput Format
Performed secondary screening of compounds identified from the 20000 screening campaign
2) Title: Anti-bacterial Project (Helicase - DnaB) for the development of a broad-spectrum anti- bacterial for use in hospital infection & community care (Dec’99-Mar’01)
Team Size: 3
Scope: The project targeted the E. Coli Helicase–DnaB, Primary Replicate Helicase - The goal was to express DnaB in a soluble form and develop a gel based radioactive assay for the same which could later be used to screen potential anti bacterial compounds
2. Masters in Biological Sciences, Birla Institute of Technology and Science, Pilani (1989-1994)
3. Bachelors in Pharmacy, Birla Institute of Technology and Science, Pilani(1989-1994)
⦁ Awarded with the Biocontribute Award (Team Award) in Dec’03; the team launched a therapeutic molecule for diabetes in market in Nov’04.
⦁ Filed 2 patents in US,1 European patent(14 countries) and 2 Indian patents filed for manufacture of oral insulin.
⦁ One of my students Rohit Shankar secured admission into Cornell University based on my recommendation. Others secured admission into Indiana University and Carnegie Mellon University.
⦁ Passed 3 courses in Courseera-Learning How to Learn: Powerful mental tools to help you master tough subjects (McMaster University & University of California San Diego);Get Organised:How to be a together Teacher(Relay Graduate School of Education); The Challenges of Global Health( Duke University)
⦁ Attended Workshop on Chromatographic separations conducted by BIO-RAD at UICT, Mumbai,2004
⦁ Attended Fast-Trac Workshop on Chromatography conducted by GE-Healthcare at Kuala Lumpur, Malaysia, 2005
⦁ Passed ISO 14000 Lead auditor course of NQA conducted by Choda Leadership Centre, Hyderabad ,July 2006
⦁ Attended Millipore Filtration School ,2007
⦁ Passed KSET exam for Lecturer in Life Sciences, 2018
⦁ Gautam Krishnan and Utpal Roy, “A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 2019, Article ID 2532045, 18 pages, 2019. https://doi.org/10.1155/2019/2532045.
https://www.ncbi.nlm.nih.gov/pubmed/31031872
⦁ Krishnan, G., Roy, U. (2019). Role of Molecular Interactions and Oligomerization in Chaperone Activity of Recombinant Acr from Mycobacterium tuberculosis. Iranian Journal of Biotechnology, 17(3), 55-62. doi: 10.29252/ijb.2370
⦁ Krishnan G and Roy U. Prediction of recombinant Mycobacterium tuberculosis α-crystallin oligomer chaperone activity using polynomial graphs]. F1000Research 2020, 7:1801 (https://doi.org/10.12688/f1000research.16328.2)
⦁ Title of paper “Cloning and expression of antigenic proteins of pathogenic organisms: special focus on Mycobacterial family” by Dr Utpal Roy, Gautam Krishnan, Sam C., Joy B., Natesh, Siddharth T., Rohit Shivkumar presented at NATCON 2014 – Feb 7th, 2015, Mumbai
⦁ Conference presentation: Key note speaker “A functional model for predicting in vitro chaperone activity of M. tb Acr” at 3rd International Conference of Clinical Microbiology, Kyoto, Japan, May 1st - 2nd, 2019 Clin Microbiology,2019,Vol 8 doi 10.4172/2327-5073-C2-047
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15 Reviews
78 Students
1 Course
Latest Reviews
3 years ago
SHREYA MUTHUKUMAR
I just completed the module! First of all thank you for letting me take this course for free???? I really enjoyed the course...the pace was optimal and I liked the fact that you had taken examples from your own work and shared a lot of your personal experience.
I could actually think of so many things that could have gone wrong with our the pcr reaction we had done in college.